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hand = guidata(untitled);
object = hand.pushbutton1;
eventdata = [];
pushbutton1_Callback(object, eventdata, hand);

function varargout = untitled(varargin)

% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...
                   'gui_Singleton',  gui_Singleton, ...
                   'gui_OpeningFcn', @untitled_OpeningFcn, ...
                   'gui_OutputFcn',  @untitled_OutputFcn, ...
                   'gui_LayoutFcn',  [] , ...
                   'gui_Callback',   []);
if nargin && ischar(varargin{1})
    gui_State.gui_Callback = str2func(varargin{1});
end

if nargout
    [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
    gui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT


% --- Executes just before untitled is made visible.
function untitled_OpeningFcn(hObject, eventdata, handles, varargin)


% Choose default command line output for untitled
handles.output = hObject;

% Update handles structure
guidata(hObject, handles);

% UIWAIT makes untitled wait for user response (see UIRESUME)
% uiwait(handles.figure1);


% --- Outputs from this function are returned to the command line.
function varargout = untitled_OutputFcn(hObject, eventdata, handles)
% varargout  cell array for returning output args (see VARARGOUT);

% Get default command line output from handles structure
varargout{1} = handles.output;


% --- Executes on button press in pushbutton1.
function pushbutton1_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
set(handles.pushbutton1, 'Enable', 'off');
set(handles.pushbutton2, 'Enable', 'on');

% --- Executes on button press in pushbutton2.
function pushbutton2_Callback(hObject, eventdata, handles)
% hObject    handle to pushbutton2 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
set(handles.pushbutton1, 'Enable', 'on');
set(handles.pushbutton2, 'Enable', 'off');